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  • Quantitative Proteomic Analysis of Optimal Cutting ...

    Tumor tissue preservation in optimal cutting temperature (OCT) compound is commonly used in the surgical suite. However, proteins recovered from OCT-embedded specimens pose a challenge for LC-MS/MS experiments, due to the large amounts of polymers present in OCT.

  • Quantitative glycoproteomic analysis of optimal cutting ...

    Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive prostate cancer Anal Chem. 2011 Sep 15;83(18):7013-9. doi: 10.1021/ac200815q. Epub 2011 Aug 18. Authors Yuan Tian 1 ...

  • Optimal cutting temperature medium embedding and cryostat ...

    Jul 05, 2020 · To maximize data obtainment from valuable cardiac tissue, we hypothesized that myocardium fixed in optimal cutting temperature (OCT) medium for histology could also be used to investigate the function of myofilament proteins in situ. We compared tissue prepared via conventional liquid nitrogen (LN) snap freezing with tissue fixed in OCT and ...

  • Removal of optimal cutting temperature (O.C.T.) compound ...

    Feb 09, 2021 · Up to10%cash back · Firstly, the common embedding of samples in optimal cutting temperature (O.C.T.), which contains high concentrations of polyethylene glycol (PEG) polymers, causes analyte signal suppression during mass spectrometry (MS) …

  • CUTTING TEMPERATURE MEASUREMENT AND MATERIAL …

    Values of maximum cutting temperature measured with natural thermocouple are up to 600 o C [23, 26]. With application of artificial thermocouples placed in the work-piece,

  • Fresh‐frozen, optimal cutting temperature (OCT) compound ...

    Feb 19, 2010 · The usefulness of fresh‐frozen, optimal cutting temperature (OCT) compound‐embedded (FFOE) bone marrow (BM) aspirates was evaluated as a reliable resource for morphological, immunohistochemical and molecular examinations. One hundred BM aspirates were collected in polypropylene tubes and immediately frozen for 2 h in a deep freezer.

  • Does Temperature Affect Rooting of Cuttings? | Home Guides ...

    Cuttings and Temperature. It is important to note that soil temperature, not air temperature, is the critical factor in rooting. Cuttings of most plants root best when the rooting medium is at ...

  • Determination of a coupling equation for milling ...

    May 27, 2017 · The optimization of cutting parameters has a great concern in the field of manufacturing process, especially the relationships among cutting parameters, with the study aiming at exploring a coupling equation to cutting parameters based on the optimal cutting temperature in the milling process of precipitation-hardening stainless steel with cemented carbide inserts.

  • Optimal cutting temperature compound | definition of ...

    optimal cutting temperature compound: An embedding medium for preparing tissues for sectioning and microscopic analysis. See also: compound

  • Optimal cutting temperature compound | Article about ...

    Looking for optimal cutting temperature compound? Find out information about optimal cutting temperature compound. in chemistry, a substance composed of atoms atom , basic unit of matter; more properly, the smallest unit of a chemical element having the properties of... Explanation of optimal cutting temperature compound

  • Can You Stand the Cold? Cryosectioning for Beginners

    Jan 18, 2017 · When the tissue is too cold it can smudge and will not be a thin slice. The recommended chamber temperature is -20 0 C. However, if you keep your microtome spindle (which holds the tissue block, Figure 2) at -20 0 C, then you should keep your chamber temperature just slightly colder at about -22 0 C or -23 0 C.

  • MATERIAL SAFETY DATASHEET - Ted Pella

    (NOTE: O.C.T. stands for Optimal Cutting Temperature) Synonym(s): N/A Recommended Uses: N/A Manufactured For: Sakura Finetek USA, Inc. Emergency Telephone Number: Torrance, CA 90501 USA 800-424-9300 (Chemtrec) Tel: 310-972-7800 2. Hazards Identification EMERGENCY OVERVIEW DANGER: None determined. Health Effects: None determined.

  • Optimal cutting temperature compound synonyms, optimal ...

    Synonyms for optimal cutting temperature compound in Free Thesaurus. Antonyms for optimal cutting temperature compound. 71 synonyms for compound: combination, mixture ...

  • Cryosectioning Tissues

    OCT is viscous at room temperature and miscible with H 2 O, but freezes into a solid support at −20°C. Certain soft tissues, such as brain, are optimally frozen in M-1 medium at −3°C. 2. Cut sections 5-15 μm thick in the cryostat at −20°C.

  • Tissue-Plus O.C.T. CompoundTissue-Plus O.C.T. Compound ...

    Description Embedding medium for frozen tissue specimens to ensure optimal cutting temperature (O.C.T) Water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at... Can be frozen between −10 and −80°C Will not dull cryostat blades Leaves no ...

  • OCT - Optimal Cutting Temperature - All Acronyms

    stop animation stop. slide 2 of 3. OCT is a shorter form of Optimal Cutting Temperature. OCT means Optimal Cutting Temperature. OCT is an acronym for Optimal Cutting Temperature. Search for abbreviation meaning, word to abbreviate, or category. Shortcuts for power users - examples.

  • Cutting Temperature - an overview | ScienceDirect Topics

    The temperature of the end milling cutter rises rapidly with an increase in cutting speed, and it is approximately 770 °C at 628 m min −1.

  • Fresh‐frozen, optimal cutting temperature (OCT) compound ...

    Feb 19, 2010 · The usefulness of fresh-frozen, optimal cutting temperature (OCT) compound-embedded (FFOE) bone marrow (BM) aspirates was evaluated as a reliable resource for morphological, immunohistochemical and molecular examinations. One hundred BM aspirates were collected in polypropylene tubes and immediately frozen for 2 h in a deep freezer.

  • Optimal cutting temperature compound legal definition of ...

    Definition of optimal cutting temperature compound in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is optimal cutting temperature compound? Meaning of optimal cutting temperature compound as a legal term. What does optimal cutting temperature compound …

  • Comparative LC–MS/MS lipidomic analysis of macaque heart ...

    Jun 25, 2021 · Optimal cutting temperature (OCT) blends have shown to provide stability to the embedded tissue and is compatible with spectroscopic methods, such as infrared (IR) and Raman spectroscopy. Data derived from omics-methods are only useful if tissue damage caused by storage in OCT is minimal and well understood.

  • Does Temperature Affect Rooting of Cuttings? | Home Guides ...

    Cuttings of most plants root best when the rooting medium is at about 70 degrees, though temperatures as low as 55 degrees are acceptable. Heating the rooting medium above 75 degrees isn't...

  • Optimal Cutting Temperature OCT compound - Agar Scientific

    ADD TO QUOTE. Product Description. OCT Compound is a water-soluble blend of glycols and resins that provides a convenient specimen matrix for cryostat sectioning at temperatures of -10˚C and below. OCT leaves no residue on slides during staining procedure, eliminating undesirable background staining. Dissected tissue, mounted in OCT Compound can be frozen between -20 and -80˚C.

  • Removal of optimal cutting temperature (O.C.T.) compound ...

    Feb 09, 2021 · Firstly, the common embedding of samples in optimal cutting temperature (O.C.T.), which contains high concentrations of polyethylene glycol (PEG) polymers, causes analyte signal suppression during mass spectrometry (MS) by competing for available ions during ionisation.